Botanical characteristics, matK sequence and preliminary chemical composition of Manuka - Leptospermum scoparium, Myrtaceae
Các tác giả
DOI: https://doi.org/10.59294/HIUJS2025028Từ khóa:
Leptospermum scoparium, matK, morphology, anatomy, phytochemical constituentsTóm tắt
Background: Leptospermum scoparium is an introduced plant, used as an ornamental in Vietnam. Leptospermum scoparium has anti-inflammatory, antibacterial effects, treats cough, fever, and diarrhea. Objectives: The aim of this study is to investigate the botanical characteristics, matK sequence for identification of Manuka, and preliminary phytochemical screening. Materials and Methods: Fresh trees collected in Bao Loc - Lam Dong; the morphology was evaluated through detailed observation of fresh stem, leaf, and flower specimens. Anatomical studies involved preparing cross-sections with a razor, staining them with carmine and iodine green, and capturing microphotographs using a Samsung digital camera mounted on the microscope. The matK sequence was examined, and the chemical composition of the stem and leaf powder was analyzed using the modified Ciulei method. Results: L. scoparium was identified as Leptospermum scoparium J.R. Forst. & G.Forst., based on morphological characteristics and matK sequence with the following featured morphological characteristics: Simple leaf, alternate, 5 sepals free, 5 petals free, the subpart of the petals is present, numerous stamen arranged in the same circle, 5 carpels, inferior ovary and data of anatomy: Sclereid in endodermis; inner phloem, secretory bags and calci oxalate crystals in the cross-section of the stems and leaves, parenchyma tissue in the cross-section of the leaves have a symmetrical structure; phytochemical constituents of the stems and leaves include: Essential oil, carotenoids, triterpenoids, tannins, reducing agents and organic acids; additionally, the leaves contain anthraquinones, flavonoids, and saponins. Conclusion: The study contributed to providing more data about of morphological characteristics, together with the anatomical characteristics of stems, leaves and the chemical composition of the stems, leaves of Manuka in Vietnam.
Abstract
Background: Leptospermum scoparium is an introduced plant, used as an ornamental in Vietnam. Leptospermum scoparium has anti-inflammatory, antibacterial effects, treats cough, fever, and diarrhea. Objectives: The aim of this study is to investigate the botanical characteristics, matK sequence for identification of Manuka, and preliminary phytochemical screening. Materials and Methods: Fresh trees collected in Bao Loc - Lam Dong; the morphology was evaluated through detailed observation of fresh stem, leaf, and flower specimens. Anatomical studies involved preparing cross-sections with a razor, staining them with carmine and iodine green, and capturing microphotographs using a Samsung digital camera mounted on the microscope. The matK sequence was examined, and the chemical composition of the stem and leaf powder was analyzed using the modified Ciulei method. Results: L. scoparium was identified as Leptospermum scoparium J.R. Forst. & G.Forst., based on morphological characteristics and matK sequence with the following featured morphological characteristics: Simple leaf, alternate, 5 sepals free, 5 petals free, the subpart of the petals is present, numerous stamen arranged in the same circle, 5 carpels, inferior ovary and data of anatomy: Sclereid in endodermis; inner phloem, secretory bags and calci oxalate crystals in the cross-section of the stems and leaves, parenchyma tissue in the cross-section of the leaves have a symmetrical structure; phytochemical constituents of the stems and leaves include: Essential oil, carotenoids, triterpenoids, tannins, reducing agents and organic acids; additionally, the leaves contain anthraquinones, flavonoids, and saponins. Conclusion: The study contributed to providing more data about of morphological characteristics, together with the anatomical characteristics of stems, leaves and the chemical composition of the stems, leaves of Manuka in Vietnam.
Tài liệu tham khảo
[1] R. D. Kannangara, “Identification of Leptospermum scoparium essential oils and their genetic control”, Agricultural Science, 2020.
[2] N. G. Porter and A. L Wilkins, “Chemical, physical and antimicrobial properties of essential oils of Leptospermum scoparium and Kunzea ericoides”, Phytochemistry, vol. 50, 3, 407-415, 1999. DOI: 10.1016/S0031-9422(98)00548-2.
DOI: https://doi.org/10.1016/S0031-9422(98)00548-2[3] E. Mavric, S. Wittmann,...and T. Henle, “Identification and quantification of methylglyoxal as the dominant antibacterial constituent of Manuka (Leptospermum scoparium) honeys from New Zealand”, Molecular Nutrition Food Research, vol. 52, 4, 2008. DOI: 10.1002/mnfr.200700282.
DOI: https://doi.org/10.1002/mnfr.200700282[4] W. Xu, D, Shi,...and D. G. Popovich, “TLC-Bioautography-Guided Isolation and Assessment of Antibacterial Compounds from Manuka (Leptospermum scoparium) Leaf and Branch Extracts”, Selected Scholars' Exclusive Papers on Natural Products Chemistry, vol. 29, 3, 717, 2024. DOI: 10.3390/molecules29030717.
DOI: https://doi.org/10.3390/molecules29030717[5] N. T. T. Hằng, Giáo trình Thực tập thực vật dược, Đại học Y Dược Thành phố Hồ Chí Minh, 2023.
[6] Florabase, “Leptospermum scoparium” 2024. [Online]. Available: https://florabase.dbca.wa.gov.au/
browse/profile/21833 [Accessed July 17, 2024]
[7] The World Flora Online, “Leptospermum scoparium” 2024. [Online]. Available: https://www.worldfloraonline.org/taxon/wfo-0000226751 [Accessed June 25, 2024].
[8] C.J. Webb and W. R. Sykes, PJ GJ. Flora of New Zealand. Vol IV: Naturalised pteridophytes, gymosperms, dicotyledons. 1988.
[9] J. Yu, J. H. Xue and S. L. Zhou, “New universal matK primers for DNA barcoding angiosperms”, Journal of Systematics and Evolution, vol. 49, 3, 176-181, 2011. DOI: 10.1111/j.1759-6831.2011.00134.x.
DOI: https://doi.org/10.1111/j.1759-6831.2011.00134.x[10] T. Hùng và T. T. V. Anh, Phương pháp nghiên cứu dược liệu, Đại học Y Dược Thành phố Hồ Chí Minh, 2020.
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