Establishment of quality standards for Morinda citrifolia L. Rubiaceae harvested at Mephydica Co., LTD.
Các tác giả
DOI: https://doi.org/10.59294/HIUJS2026073Từ khóa:
Morinda citrifolia, total flavonoid content, total polyphenol, scopoleƟn, UV-VisTóm tắt
Background: Noni fruit (Morinda citrifolia) is widely used in tradiƟonal medicine for health support; however, quanƟtaƟve scienƟfic data on its acƟve consƟtuents remain limited. Previous studies have shown that flavonoids, polyphenols, coumarins, and scopoleƟn contribute to the biological acƟviƟes of Noni fruit, but their contents are strongly influenced by raw material origin, ripeness, and processing methods. The absence of quality and dosage standardizaƟon may affect therapeuƟc effecƟveness and safety. ObjecƟve: This study aimed to perform preliminary phytochemical screening, thin-layer chromatography (TLC), and quanƟtaƟve determinaƟon of total flavonoids, total polyphenols, and scopoleƟn in Noni fruit. Materials and methods: Noni fruits were collected from Mephydica Co., Ltd. The chemical composiƟon was evaluated using preliminary phytochemical screening and TLC. Total flavonoid content (TFC) and total polyphenol content (TPC) were determined using the aluminum chloride and Folin-Ciocalteu methods, respecƟvely. ScopoleƟn was quanƟfied by UV-Vis spectrophotometry. Results: Phytochemical screening revealed the presence of diverse bioacƟve compounds, including coumarins, saponins, and reducing agents. TLC analysis showed a chromatographic spot corresponding to the scopoleƟn reference standard. The TFC of the methanolic extract was 25.232 ± 0.023 mg QE/g dry extract, while the TPC was 6.413 ± 0.112 mg GAE/g dry extract. The scopoleƟn content was determined as 1.636 ± 0.05 mg/g dry extract. Conclusion: The study provides quanƟtaƟve data on flavonoids, polyphenols, and scopoleƟn in methanolic extracts of Noni fruit, supporƟng its tradiƟonal use and contribuƟng to quality standardizaƟon and future applied research.
Abstract
Background: Noni fruit (Morinda citrifolia) is widely used in tradiƟonal medicine for health support; however, quanƟtaƟve scienƟfic data on its acƟve consƟtuents remain limited. Previous studies have shown that flavonoids, polyphenols, coumarins, and scopoleƟn contribute to the biological acƟviƟes of Noni fruit, but their contents are strongly influenced by raw material origin, ripeness, and processing methods. The absence of quality and dosage standardizaƟon may affect therapeuƟc effecƟveness and safety. ObjecƟve: This study aimed to perform preliminary phytochemical screening, thin-layer chromatography (TLC), and quanƟtaƟve determinaƟon of total flavonoids, total polyphenols, and scopoleƟn in Noni fruit. Materials and methods: Noni fruits were collected from Mephydica Co., Ltd. The chemical composiƟon was evaluated using preliminary phytochemical screening and TLC. Total flavonoid content (TFC) and total polyphenol content (TPC) were determined using the aluminum chloride and Folin-Ciocalteu methods, respecƟvely. ScopoleƟn was quanƟfied by UV-Vis spectrophotometry. Results: Phytochemical screening revealed the presence of diverse bioacƟve compounds, including coumarins, saponins, and reducing agents. TLC analysis showed a chromatographic spot corresponding to the scopoleƟn reference standard. The TFC of the methanolic extract was 25.232 ± 0.023 mg QE/g dry extract, while the TPC was 6.413 ± 0.112 mg GAE/g dry extract. The scopoleƟn content was determined as 1.636 ± 0.05 mg/g dry extract. Conclusion: The study provides quanƟtaƟve data on flavonoids, polyphenols, and scopoleƟn in methanolic extracts of Noni fruit, supporƟng its tradiƟonal use and contribuƟng to quality standardizaƟon and future applied research.
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