Survey of botanical characteristics and DNA barcode of Sacha inchi (Plukenetia volubilis L.) - Euphorbiaceae
DOI:
https://doi.org/10.59294/HIUJS2026032Keywords:
Sachi, botanical characteristics, DNA barcoding, ITS2, Plukenetia volubilis L.Abstract
Background: Sacha inchi (Plukenetia volubilis L.), a species originating from the Amazon rainforest and widely cultivated in Peru, is well recognized for its high nutritional and medicinal value. However, comprehensive studies on microscopic anatomy and the microscopic characteristics of powdered drugs remain limited. Objective: This study aims to examine the botanical characteristics and conduct DNA barcoding of Plukenetia volubilis to support accurate species authentication. Materials and methods: Sacha inchi samples were collected from the Medicinal Plant Garden of Mien Dong Innovative Technology University in May 2024. Morphological description and anatomical examination were carried out following standard botanical procedures. Powder microscopy was performed to observe diagnostic constituents. DNA barcoding was conducted using the ITS2 region. Results: Based on morphological traits, anatomical structures, and ITS2 DNA sequence analysis, the collected specimen was identified as Plukenetia volubilis L., belonging to the family Euphorbiaceae. Powder microscopy revealed characteristic components, including parenchyma fragments, spiral vessels, scalariform vessels, spherical - spiny calcium oxalate crystals, stomata, and non-glandular hairs. Conclusion: The study contributes to the accurate identification of Plukenetia volubilis L. and provides data to support species identification and quality control in subsequent studies.
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