Development and validation of a High-Performance Liquid Chromatography method for quantification of total oleanolic acid in Achyranthes aspera L.
Các tác giả
DOI: https://doi.org/10.59294/HIUJS2026075Từ khóa:
Achyranthes aspera, Oleanolic Acid, HPLC-DAD, quanƟtaƟve analysis, validaƟonTóm tắt
Background: Achyranthes aspera L. is a medicinal plant commonly found in Vietnam, tradiƟonally used for its cooling properƟes and therapeuƟc effects on the liver and kidneys, including relieving consƟpaƟon, reducing blood pressure, and treaƟng hepaƟƟs and polyarthriƟs. The major bioacƟve consƟtuents include saponins, alkaloids, flavonoids, and oleanolic acid - a triterpenoid considered a potenƟal marker compound for quality control. ObjecƟve: This study aimed to establish and validate a reliable analyƟcal procedure for quanƟfying total oleanolic acid in A. aspera to enhance the standardizaƟon of its quality assessment. Method: Chromatographic separaƟon was achieved on a Shim-pack GIST C18 column (250 × 4.6 mm, 5 μm) using a Shimadzu LC-2030C 3D Plus system. An isocraƟc mobile phase of acetonitrile and 0.1% phosphoric acid (80:20, v/v) was employed, and detecƟon was performed at 190 nm. The column temperature was maintained at 40°C, with an injecƟon volume of 20 μL and a total run Ɵme of 20 minutes. Results: The method exhibited excellent linearity over 0.51 - 147.4 µg/mL (R² = 0.9995). Accuracy, confirmed by recovery tests, ranged from 97.03% to 101.22%, and precision was confirmed with a relaƟve standard deviaƟon (RSD) of 3.3%, demonstraƟng the method's reliability and suitability for the quanƟficaƟon of oleanolic acid in Achyranthes aspera. Conclusion: the validated HPLC-DAD method provides a precise, sensiƟve, and reproducible tool for the quality control and standardizaƟon of Achyranthes aspera, facilitaƟng its applicaƟon in research and the development of standardized herbal products.
Abstract
Background: Achyranthes aspera L. is a medicinal plant commonly found in Vietnam, tradiƟonally used for its cooling properƟes and therapeuƟc effects on the liver and kidneys, including relieving consƟpaƟon, reducing blood pressure, and treaƟng hepaƟƟs and polyarthriƟs. The major bioacƟve consƟtuents include saponins, alkaloids, flavonoids, and oleanolic acid - a triterpenoid considered a potenƟal marker compound for quality control. ObjecƟve: This study aimed to establish and validate a reliable analyƟcal procedure for quanƟfying total oleanolic acid in A. aspera to enhance the standardizaƟon of its quality assessment. Method: Chromatographic separaƟon was achieved on a Shim-pack GIST C18 column (250 × 4.6 mm, 5 μm) using a Shimadzu LC-2030C 3D Plus system. An isocraƟc mobile phase of acetonitrile and 0.1% phosphoric acid (80:20, v/v) was employed, and detecƟon was performed at 190 nm. The column temperature was maintained at 40°C, with an injecƟon volume of 20 μL and a total run Ɵme of 20 minutes. Results: The method exhibited excellent linearity over 0.51 - 147.4 µg/mL (R² = 0.9995). Accuracy, confirmed by recovery tests, ranged from 97.03% to 101.22%, and precision was confirmed with a relaƟve standard deviaƟon (RSD) of 3.3%, demonstraƟng the method's reliability and suitability for the quanƟficaƟon of oleanolic acid in Achyranthes aspera. Conclusion: the validated HPLC-DAD method provides a precise, sensiƟve, and reproducible tool for the quality control and standardizaƟon of Achyranthes aspera, facilitaƟng its applicaƟon in research and the development of standardized herbal products.
Tài liệu tham khảo
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